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  • Endometrial Cancer br conjugates c u br conjugates

    2020-08-12

    Endometrial Cancer
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    Indicator Ovarian Cancer
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    Table 5 Spearman correlation coefficients between the biochemical composition of saliva and IR spectroscopy data (p < 0.05).
    Fig. 4. Results of discriminant analysis (A - only biochemical composition of saliva, B - only IR spectroscopy data, C - combined biochemistry and IR spectroscopy data). In figure: 1 - endometrial cancer, 2 - ovarian cancer, 3 - control group.
    The data obtained are consistent with the literature, according to which the important difference between malignant and non-malignant tissue lies in the lipid region of the spectrum [43]. Gajjar et al. in-vestigated plasma and serum in combination with classification methods for the detection of ovarian cancer [41,78,79]. The results show that lower levels of lipids are observed for borderline serous tu-mors, especially the asymmetric stretching bands of CH3 (2955 cm−1) and the symmetric stretching bands of CH2 (2850 cm−1). In the present work, for the saliva of patients with ovarian cancer, the lowest values of the intensity of the Melatonin bands of 2853 and 2957 cm−1 are noted (Table 1, Fig. 2C and E). Petibois et al. suggested calculating a ratio of 2920/2955 cm−1 for differentiation of border samples as compared to malignant ones [80]. We have shown that the ratio of 2923/2957 cm−1 regularly decreases in the transition from the control group to non-malignant pathologies of the endometrium and ovaries, and then to cancer pathologies, reaching the maximum difference with the control 
    at advanced stages of the disease (Fig. 3). This allows using this ratio as a potentially informative marker in the clinical laboratory diagnosis of ovarian and endometrial cancer. This is especially true since today there are no highly sensitive and specific diagnostic markers for en-dometrial cancer [81–83].
    The limitations of the study include the small amount of data in each group, which does not allow for differences in the phenotype of tumors. Interesting, in our opinion, is the comparison of the obtained data with the level of tumor markers used in traditional laboratory diagnostics of ovarian cancer (CA-125 + HE4). Potentially informative may be the study of the absorption bands of proteins and nucleic acids on the IR spectra of saliva without preliminary extraction of lipids, as well as a comprehensive assessment of changes in the lipid, protein profile and DNA/RNA ratio using IR spectroscopy in combination with multidimensional statistical analysis.
    5. Conclusions
    Thus, the possibility of determining changes in lipid levels in ovarian and endometrial cancer using saliva has been shown. A mod-ification of the Folch method for pre-extraction of lipids with sub-sequent analysis of samples by IR spectroscopy was proposed. It has been established that the intensity ratio of 2923/2957 cm−1 is statis-tically significantly reduced in the pathologies of the ovaries and the endometrium in both the early and advanced stages of the disease, which can be used as a new diagnostic criterion. Additionally, there was a violation of lipid peroxidation processes in cancer, which can also be assessed by the IR spectra of saliva lipid extracts. The combination of spectroscopic data and the results of biochemical analysis of saliva improves the accuracy of differentiation of the studied groups, which represents a promising direction in the search for new diagnostic markers of ovarian and endometrial cancer.
    References
    9 Accepted Manuscript
    Analysis of the National Cancer Database Esophageal Squamous Cell Carcinoma in the United States
    Brandon C. Chapman, MD, Michael Weyant, MD, Sarah Hilton, MD, Patrick W. Hosokawa, BS, Martin D. McCarter, MD, Ana Gleisner, MD, PhD, Nader D. Nader, MD, PhD, Csaba Gajdos, MD.
    To appear in: 
    The Annals of Thoracic Surgery
    Please cite this article as: Chapman BC, Weyant M, Hilton S, Hosokawa PW, McCarter MD, Gleisner A, Nader ND, Gajdos C, Analysis of the National Cancer Database Esophageal Squamous Cell Carcinoma in the United States, The Annals of Thoracic Surgery (2019), doi: https://doi.org/10.1016/ j.athoracsur.2019.05.053.
    This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
    ACCEPTED MANUSCRIPT
    Analysis of the National Cancer Database Esophageal Squamous Cell Carcinoma in the United States